DETERMINATION OF INTERACTION PRODUCTS OF SELENITE SODIUM AND AMINO ACID SELENOCYSTINE WITH REDUCED GLUTATHIONE BY HPLC METHOD 

Poluboyarinov Pavel Arkadyevich, Candidate of agricultural sciences, associate professor, sub-department of phisics and chemistry, Penza State University of Architecture and Construction (28 G. Titova street, Penza, Russia), poluboyarinovpavel@yandex.ru
Moiseeva Inessa Yakovlevna, Doctor of medical scienses, professor, head of sub-department of general and clinical pharmacology, dean of the faculty of general medicine, Penza State University (40 Krasnaya street, Penza, Russia), moiseeva_pharm@mail.ru
Glebova Natali Nikolaevna, Senior lecturer, sub-department of general and clinical pharmacology, Penza State University (40 Krasnaya street, Penza, Russia), natali.glebova@gmail.com

543.621, 543.641

10.21685/2307-9150-2016-4-7

Background. Selenium is a necessary microelement in organisms of animals and humans. Selenocystine is an amino acid being the most optimal form for selenium deficiency correction. The purpose of the work is to study reaction products betweenamino acid selenocystine or sodium selenite and reduced glutathione by HPLC method.
Materials and methods. The course of reactions between sodium selenite or selenocystine and reduced glutathione at various рН values was controlled by HPLC method a “Milichrom A-02” chromatograph (CJSC EkoNova).
Results. The paper describes reaction products of sodium selenite and reduced glutathione in various molar ratios and at рН values studied by HPLC method. The formation of a reaction intermediate product – glutathione selenium disulfide – has been revealed and its absorption spectrum in stopped flow has been obtained. Selenocystine interacts with reduced glutathione and forms selenocysteine-glutathione selenium sulfide, instead of hydroselenide-anion, that confirms the data about its enzymatic metabolism in biological media.
Conclusions. Selenocystine amino acid interacting with reduced glutathione forms only an intermediate product – selenocysteine-glutathione selenium sulfide – unlike sodium selenite, which is reduced to hydroselenide-anion.

sodium selenite, selenocystine, reduced glutathione, glutathione selenium disulfide, hydroselenide-anion, selenocysteine-glutathione selenium sulfide, HPLC

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